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EXP-PAK™ Cell Expansion Bio-Containers

Charter Medical’s EXP-Pak™ Bio-Containers are intended for the expansion and culture of non-adherent cells. The container is made from a single-web polyolefin gas permeable film. The integrated tubing allows for functionally closed system filling, feeding, and sampling.  EXP-Pak™ can be used to harvest, expand and transfer cell cultures typically used in cellular therapy applications.

Features and Benefits

  • Cell Expansion Observed with Multiple Cell Models
  • Gas Permeable Film
  • Reusable Sampling Valve for In-Process Testing
  • Tubing for sterile weld Connections
  • Scalable, User Friendly Design

 

Download the EXP-Pak™ Data Sheet

Cell Expansion

EXP-Pak™ containers were evaluated for their ability to promote expansion of cells in suspension. Th2 cell cultures were initiated with CD4+ selected lymphocytes and placed into culture in X-Vivo 20 media (Lonza) containing 5% HI autologous or HI AB plasma, 20 IU/mL IL-2 (Chiron), 1000 IU/mL IL-4 (Cell Genix) and 1uM rapamycin (Sirolimus) in the EXP-Pak™ bag. Cells were stimulated on Day 0 with 3:1 (beads per cell) anti-CD3 and anti-CD28 coated beads (4.5uM Dynal tosylactivated). The data demonstrated cell expansion over a 10 day culture period.

Download the EXP-Pak™ White Paper

Ordering Information – EXP-Pak™ Bio-Containers

Product Number Description Case Qty
 EXP-50  30 mL fill volume at 1 cm thick cell expansion bag with two leads and female luers, and dockable fill line with sampling valve  24
EXP-500 190 mL fill volume at 1 cm thick cell expansion bag with two leads and female luers, and dockable fill line with sampling valve 12
EXP-1L 380 mL fill volume at 1 cm thick cell expansion bag with two leads and female luers, and dockable fill line with sampling valve 12
EXP-3L 630 mL fill volume at 1 cm thick cell expansion bag with two leads and female luers, and dockable fill line with sampling valve  12
EXP-5L 1250 mL fill volume at 1 cm thick cell expansion bag with two lead and female luers, with sampling valve 12